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N2913
Sigma
N-TER™ Nanoparticle siRNA Transfection System
Description
| Application | The N-TER Nanoparticle siRNA Transfection System is a peptide-based system for use in the transient knockdown of eukaryotic gene expression. The N-TER Peptide binds siRNAs non-covalently, forming a nanoparticle. This nanoparticle interacts directly with lipids on the surface of the plasma membrane, allowing the nanoparticle to diffuse across the cell membrane and deliver the siRNA directly to the cytoplasm. Unlike most lipid-based transfection methods, this novel delivery mechanism bypasses the endosomal pathway, eliminating possible compartmentalization and degradation of the siRNA. |
| Legal Information | Manufactured and Distributed by Sigma-Aldrich, under license from the CNRS. |
| N-TER is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| shipped in | wet ice |
| storage temp. | −20°C |
References
| reference | Simeoni F, et al., Insight into the mechanism of the peptide-based gene delivery system MPG: implications for delivery of siRNA into mammalian cells Nucleic Acids Res. 31, 2717-2724, (2003) Abstract |
| Crombez L, et al., A non-covalent peptide-based strategy for siRNA delivery Biochem. Soc. Trans. 35, 44-46, (2007) Abstract | |
| Deshayes S, et al., Delivery of proteins and nucleic acids using a non-covalent peptide-based strategy Adv. Drug Delv. Rev. 60, 537-547, (2008) Abstract |
